ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Biochemical constituent, enzyme inhibitory activity, and molecular docking analysis of an endemic plant species, Thymus migricus

Abdülmelik Aras, Fikret Türkan, Umit Yildiko, Mehmet Nuri Atalar, Ömer Kılıç, Mehmet Hakki Alma, and Ercan Bursal

Department of Biochemistry, Faculty of Science and Arts, Iğdır University, Iğdır, Turkey

E-mail: e.bursal@alparslan.edu.tr

Received: 4 August 2020  Accepted: 22 September 2020

Abstract:

Medicinal plants have been used traditionally since ancient times as alternative medications to treat various human diseases. In this work, we examined the chemical constituent, antioxidant activity, and enzyme inhibition of an endemic plant Thymus migricus. The plant extracts showed remarkable inhibition effects against the acetylcholinesterase (AChE), butyrylcholinesterase (BChE), glutathione S-transferase (GST), and α-glycosidase (α-Gly) enzymes that are linked with some metabolic disorders. IC₅₀ values (concentration of a sample to inhibit 50% of enzyme activity) for AChE, BChE, GST, and α-Gly were found as 18.23 mg/mL, 17.77 mg/mL, 31.5 mg/ml, and 16.5 mg/mL, respectively. Antioxidant activities of water extract of T. migricus (WET) and methanol extract of T. migricus (MET) were determined using four in vitro techniques. The extracts showed considerable antioxidant actions on all four techniques. In addition, quinic acid (18.30 mg/g), chlorogenic acid (0.94 mg/g), cynaroside (0.49 mg/g), luteolin (0.21 mg/g), and p-coumaric acid (0.19 mg/g) were characterized as major phenolic compounds using advanced LC–MS/MS technique. The interactions of some phenolic compounds with the enzymes have been investigated using molecular docking studies. The docking analysis results revealed that the interactions of the major compounds of the plant with AChE, BChE, GST, and α-Gly enzymes are responsible for the inhibitory activity.

Keywords: Antioxidant; Enzyme inhibition; Phenolic compounds; Thymus migricus; Docking

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-020-01375-z

Chemical Papers 75 (3) 1133–1146 (2021)