ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Development of MoS₂ and Au nanoparticle ıncluding disposable CEA-based immuno-cytosensor platforms

Derya Bal Altuntaş, Hatice S. Nalkıran, Sema Aslan, and Zuhal Yolcu

Department of Bioengineering, Faculty of Engineering and Architecture, Recep Tayyip Erdogan University, Rize, Turkey

E-mail: derya.balaltuntas@erdogan.edu.tr

Received: 29 January 2022  Accepted: 16 April 2022

Abstract:

In this study, an electrochemical biosensor for determination of Carcinoembryonic antigen (CEA) biomarker using human breast ductal adenocarcinoma (MCF-7) and human ovarian adenocarcinoma (SK-OV-3) cancer cells was presented. Disposable pencil graphite electrodes (PGE) has been modified with Au nanoparticle (Au NP) and MoS₂ nanostructure dispersed chitosan (Cs) matrix. Therefore, the electrochemical interaction between the antibody-electrode surface was facilitated. Under optimal conditions, the immunosensor exhibited high sensitivity toward CEA biomarker in the low concentration range 0.01–10 ng mL⁻¹, with the detection limit of 1.93 ng mL⁻¹ and relative standard deviation of 4.65 (n = 5). The results indicated that even very small changes in CEA concentration can be sensed with the presented system. Also, recovery of the immunosensor found as 98 ± 3% in the real sera samples containing dopamine and ascorbic acid. It has a great potential in the clinical screening of divergent cancer biomarkers. Experiments were conducted to determine the number of normal cell hGF and cancer cells adhered and attached on immuno-cytosensor surfaces. CEA-positive MCF-7 cells have shown great potential for adhesion and attachment to MoS₂/Cs/Au/Anti-CEA/CEA surface better than CEA-negative cells. The developed immuno-cytosensor exhibited very promising results for the future biosensor studies.

Keywords: Carcinoembriyonic antigen; Nanoparticle; Human breast ductal adenocarcinoma; Human ovarian adenocarcinoma; Immunosensor-cytosensor

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-022-02240-x

Chemical Papers 76 (8) 5217–5229 (2022)