ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Direct utilization of peach wastes for enhancements of lignocellulolytic enzymes productions by Pleurotus eryngii under solid-state fermentation conditions

Merve Akpinar and Raziye Ozturk Urek

Graduate School of Natural and Applied Sciences, Chemistry Department, Dokuz Eylül University, Buca-Izmir, Türkiye

E-mail: raziye.urek@deu.edu.tr

Received: 18 November 2021  Accepted: 27 June 2022

Abstract:

One of the main wastes that created environmental troubles is lignocellulosics such as fruit pulps collected in huge amounts from food industry. Peach wastes were utilized as the substrates raise ligninocellulolytic enzymes productions, namely manganese peroxidase (MnP), lignin peroxidase (LiP), carboxymethyl cellulase (CmCase), xylanase, exoglucanase, β-glucosidase (BGLA), by Pleurotus eryngii. The effects of different concentrations with different compounds, copper, iron, Tween 80, ammonium nitrate and manganese on productions of ligninocellulolytic enzymes were investigated contingent on incubation time. The optimal productions of ligninocellulolytic enzymes were attained by 5.0 g peach waste and 70 µM Cu²⁺, 18 µM Fe²⁺, 4.0 gL⁻¹ ammonium nitrate, 250 µM Mn²⁺, 0.025% (v/v) Tween 80 qua the inductivers. According to the results under optimized conditions, 6.51-, 9.52-, 1.86-, 3.17-, 1.06-, and 2.73-fold boosts were achieved for lignocellulolytic enzyme activities of MnP, LiP, CMCase, Xylanase, exoglucanase, BGLA, respectively. This study is important with regard to succeed the improvement of six-lignocellulolytic enzymes activities utilizing various inducers.

Graphical abstract

Keywords: Peach waste; Inducer; MnP; LiP; Cellulolytic enzymes; Solid-state fermentation

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-022-02356-0

Chemical Papers 76 (11) 6699–6712 (2022)