ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Comparison of spectrophotometric and HPLC methods for determination of lipid peroxidation products in rat brain tissues

M. Ďurfinová, M. Brechtlová, B. Líška, and Ž. Barošková

Department of Medical Chemistry, Biochemistry, and Clinical Biochemistry, Faculty of Medicine, Comenius University, Sasinkova 2, 811 08 Bratislava, Slovak Republic

E-mail: durfinova1@post.sk

Received: 23 October 2006  Revised: 1 March 2007  Accepted: 23 March 2007

Abstract: Two methods for determination of lipid peroxidation (LPX) products in rat brain homogenates were compared. The thiobarbituric acid (TBA) test and HPLC assay for analysis of malondialdehyde (MDA) were applied. Rat brain homogenate dissolved in tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) was mixed with TBA and H₃PO₄ and heated at 100°C to form colored complex that was extracted into butanol. No significant differences were found between the contents of TBA-reacting substances and their amount deduced from the MDA-TBA analysis. The presented results show that LPX products in brain homogenates can be determined without interferences also by the TBA test. Moreover, a survey of various methods used for the sample preparation before analysis of LPX products originating from different brain areas was made and compared with the obtained results.

Keywords: lipid peroxidation - malondialdehyde determination - HPLC - thiobarbituric acid

Full paper is available at www.springerlink.com.

DOI: 10.2478/s11696-007-0040-5

Chemical Papers 61 (4) 321–325 (2007)