ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Application of GF-AAS Methods for As3+ and As5+ Determination in Fish Products

M. Koreňovská and M. Suhaj

Food Research Institute, SK-824 75 Bratislava

E-mail: maria.korenovska@vup.sk

Received: 5 February 2004

Abstract: Methods of As3+ and As5+ toxic form determination in commerce fish products in Slovakia are described. Conditions of As3+ and As5+ extraction from sea fish products were optimized. Recovery of As3+ and As5+ extraction method was determined: at As3+ determination in fish it was 80—102 %, at As5+ determination in frozen sea fish it was 80—106 %. Portion of As3+ from total arsenic content estimated in frozen sea fish was from 3.1 to 15.3 %, and in tuna cans from 3 to 5.7 %. Portion of As5+ from total arsenic content was 0—12 % in frozen sea fish and 3.3— 6.3 % in tuna cans. GF-AAS method was used for total arsenic content and As3+ and As5+species determination. Samples were decomposed in microwave high-pressure system with decomposition mixture 4 cm3 HNO3 and 0.5 cm3 H2O2. LOD was determined at the level 0.003 mg kg−1 and LOQ at 0.006 mg kg−1. Combined uncertainty of measurement was 7.1 %. The trueness of the method was tested by determination of arsenic concentration in Tuna fish IMEP-20 reference material. The analyzed value was 4.56 mg kg−1, sw = 0.40 mg kg−1, while certified value was 4.93 mg kg−1, sw = 0.21 mg kg−1. Estimated contents of toxic arsenic forms in fish products on Slovak market were relatively low and total sum of them never exceeded 20 % portion of total arsenic content. This is in accordance with published data on content of inorganic arsenic in fish products.

Full paper in Portable Document Format: 593a153.pdf

Chemical Papers 59 (3) 153–156 (2005)