ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Quantitative MALDI-TOFMS Analysis of Amino Acids Applying Soft Modeling Methods

P. Maršálek, M. Farková, and J. Havel

Department of Analytical Chemistry, Faculty of Science, Masaryk University, CZ-611 37 Brno

E-mail: havel@chemi.muni.cz

Received: 3 July 2001

Abstract: This study demonstrates the applicability of Matrix-Assisted Laser Desorption/Ionization Timeof- Flight Mass Spectrometry (MALDI-TOFMS) in multicomponent analysis of amino acids with various chemometric methods like experimental design, partial least squares (PLS), and artificial neural networks (ANN). Several negative factors influencing reproducibility of intensities were systematically studied and their influence was eliminated. Binary mixtures of L-alanine and L-isoleucine were analyzed and evaluated using PLS and ANN. The results obtained show the possibility to use MALDI-TOFMS in quantitative analysis of amino acids.

Full paper in Portable Document Format: 563a188.pdf

Chemical Papers 56 (3) 188–193 (2002)