ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Determination of Water- and Fat-Soluble Vitamins in Different Matrices Using High-Performance Liquid Chromatography

A. Jedlička and J. Klimeš

Department of Pharmaceutical Chemistry and Drug Control, Faculty of Pharmacy, Charles University, CZ-500 02 Hradec Králové, Czech Republic

Received: 22 January 2004

Abstract: High-performance liquid chromatographic methods are the most often used methods for the determination of water-soluble vitamins (WSV) and fat-soluble vitamins (FSV). General approaches in quantification, occurring forms of vitamins, influences, which can affect stability of vitamins, necessary precautions in sample handling, pre-run sample stabilization, extractions procedures, and HPLC quantifications are mentioned and compared. This paper provides basic guidance for using HPLC in analysis of WSV and FSV. Finally, some methods for the quantification of WSV and FSV in pharmaceutical preparations, food supplements, and biological samples are reviewed.

Full paper in Portable Document Format: 593a202.pdf

Chemical Papers 59 (3) 202–222 (2005)