ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Kinetics of Ethyl Acetate Formation by Lipase in Organic Solvent and Solvent-Free System

K. Bélafi-Bakó, A. Kabiri Badr, N. Nemestóthy, U. Ehrenstein, and L. Gubicza

Research Institute of Chemical and Process Engineering, 8200 Veszprém, Hungary

E-mail: bako@mukki.richem.hu

Received: 1 August 2002

Abstract: Manufacturing of ethyl acetate by lipase in both organic solvent and solvent-free system was studied and the apparent kinetics of the reaction was analyzed following the Michaelis—Menten model. It was found that substrate (acid) inhibition occurred during the reaction, regardless of the media used. In order to characterize the kinetics, constants of the reaction (K_M, v_max, and K_I) were determined.

Full paper in Portable Document Format: 574a278.pdf

Chemical Papers 57 (4) 278–281 (2003)