ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Biosynthesis of Chloramphenicol. IVa. Isolation and Some Properties of 3-Deoxy-D-arabmo-heptulosonate-7-phosphate Synthetase (E. C. 4. 1. 2. 15) of Streptomyces sp. 3022a

B. Škárka, D. W. S. Westlake, and M. A. Pickard

Department of Microbiology and Biochemistry, Slovak Technical University, Bratislava 1

Abstract: Some properties of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthetase from crude cell-free extracts from Streptomyces sp. 3022a were studied. The modification of the method for determination of the enzyme activity is described. It was found that the dilution does not lead to the loss of the enzyme activity. The activity of this enzyme changes during the growth, the minimum was found the second day of cultivation. By its response to the inhibitors and some other compounds the 3-deoxy-D-arabino-heptulosonate-7-phosphate synthetase (DAHP-synthetase) from Streptomyces sp. 3022a is similar to the DAHP-synthetase from Bacillus subtilis.

Full paper in Portable Document Format: 241a73.pdf

Chemical Papers 24 (1) 73–77 (1970)