ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Oscillographic polarography of nucleic acids

E. Paleček

Biophysical Institute, Czechoslovak Academy of Sciences, Brno

Abstract: Nucleic acid bases (adenine, guanine, cytosine, 5-methylcytosine, thymine, uracil) and purine derivs. (hypoxanthine, xanthine, and uric acid) were investigated by a oscillopolarographic method with a.c. in the medium of M H₂SO₄, HCOONH₄, KCl, and NaOH. All these compds. give inflections on the curves dE/dt = F1(E) and the majority of them and the nucleosides and the nucleotides can be detd. alone or in the mixt. in the concn. 10^-5M-10^-6M. A highly polymerized deoxyribonucleic acid (I), prepd. from calf thymus or rat spleen, gives characteristic inflections in HCOONH₄, NaCl, NaOH, Brdicka's CoIII and NiII solns. In the CoIII and NiII solns., both protein and I can be detd. even in a great excess of I. The anodic inflection in HCOONH₄ is caused by the deoxyguanylic acid bound in the I mol. A hypothesis of the structure of I is given.

Full paper in Portable Document Format: 1411-12a798.pdf (in Czech)

Chemical Papers 14 (11-12) 798–806 (1960)