ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

The determination of ammonia in ammonium salts in the presence of urea by cadmium hydroxide. A modified method for the determination of the activity of enzymes

D. Prístavka and V. Krčméry

Slovak Technical University, Bratislava

Abstract: A method, based on the ability of urease to decomp. urea into NH₃ and (NH₄)₂CO₃, is described. NH₃ in the presence of urea is driven out from the NH₄ salts by Cd(OH)₂ into O.02N H₂SO₄. Cd(OH)₂, in 5% emulsion, drives out NH₃ quantitatively and the urea during the distn. of NH₃ is not decompd. The decompn. of urea does not occur until 9-min. boiling. Boiling during the distn. of NH₃ is quiet with no foaming and the added albumin is immediately pptd. The blank expts. show max. value of 0.35 ml. of 0.02N H₂SO₄.

Full paper in Portable Document Format: 103a183.pdf (in Slovak)

Chemical Papers 10 (3) 183–187 (1956)