ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Improved DPPH determination for antioxidant activity spectrophotometric assay

M. R. Szabo, C. Idiţoiu, D. Chambre, and A. X. Lupea

Chemical and Technological Research Centre, Food Engineering, Tourism, and Environmental Protection Faculty, “Aurel Vlaicu” University, Elena Dragoi Street 2, Arad, 310330, Romania

E-mail: ralucaszabo@rdslink.ro

Received: 3 August 2006  Revised: 1 December 2006  Accepted: 4 December 2006

Abstract: An improved procedure for determination of the residual DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical concentration was proposed taking into account the absorbance of both DPPH free radicals and DPPH nonradical (1,1-diphenyl-2-picrylhydrazine) stable form. The calculated residual DPPH free radical concentrations were compared with those obtained from a calibration curve and variation coefficients below 10 % were found.

Keywords: DPPH determination - antioxidant activity - spectrophotometric assay - free radicals

Full paper is available at www.springerlink.com.

DOI: 10.2478/s11696-007-0022-7

Chemical Papers 61 (3) 214–216 (2007)