ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

HPLCdetermination of chlorhexidine in dosage forms

K. Beneš and Z. Pavelek

Galena, n.e., CS-747 70 Opava

Abstract: The present paper describes the utilization of high-pressure liquid chromatography for determination of Chlorhexidine in complex samples of dosage forms. Chromatographic separation was performed on a cyanopropyl column using acetate buffer in the mixture of water and acetonitrile ( φ_r = 2:3) as the mobile phase. The linear dependence of the peak size on concentration of Chlorhexidine in the range of 0.01 to 0.20 mol m^-3 was ensured only when the samples were diluted with the mobile phase. Otherwise, the reproducibility of measurements decreased substantially.

Full paper in Portable Document Format: 425a601.pdf

Chemical Papers 42 (5) 601–606 (1988)